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get signal immunoreaction enhancer solution 1  (Toyobo)


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    Structured Review

    Toyobo get signal immunoreaction enhancer solution 1
    Get Signal Immunoreaction Enhancer Solution 1, supplied by Toyobo, used in various techniques. Bioz Stars score: 98/100, based on 3817 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/signal+immunoreaction+enhancer+solution+1/Can+Get+Signal/pmc13674482-280-29-35
    Average 98 stars, based on 3817 article reviews
    get signal immunoreaction enhancer solution 1 - by Bioz Stars, 2026-11
    98/100 stars

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    Incubation:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal (Toyobo Co., Ltd.), washed thrice using Tris-buffered saline-Tween-20 (TBS-T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti-phospho-ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti-ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti-α-actin antibody (Sigma-Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS-T and incubated with HRP-labeled anti-rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS-T, incubated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge-coupled device system (ImageQuant; LAS-4000mini; Cytiva).

    Article Title: Far-red-enriched ultra-long-day conditions induce constitutive FT expression and rapid flowering in radish rootstocks, promoting graft-mediated floral induction in Brassicaceae crops
    Article Snippet: After transfer, membranes were stained with Ponceau S solution (Beacle, Inc., Japan) and scanned to quantify total protein loading for normalization. .. Membranes were blocked with PBST (phosphate-buffered saline containing 0.1% Tween 20) containing 1% skim milk for 1 h at room temperature (20–25◦C), then incubated with an anti-FT antibody (Motoki et al., 2022) diluted to 300 ng mL−1 in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.). .. Subsequently, membranes were incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:20,000 dilution; Cell Signaling Technology, Inc., USA) in Can Get Signal Solution 2 (Toyobo Co., Ltd.) at room temperature.

    Article Title: Identification and characterization of a high-affinity antigen-binding fragment against intestinal bacteria from non-immunized rabbit single B cells using Ecobody technology.
    Article Snippet: Laboratory of Molecular Biotechnology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan, Center for One Medicine Innovation Translational Research (COMET), Nagoya University, Japan, Faculty of Animal Sciences, University of Mataram, Mataram, 83125 West Nusa Tenggara, Indonesia, and Department of Agrobiological Resources, Faculty of Agriculture, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya 468-8502, Japan

    Article Title: Disrupted FLOWERING LOCUS C alleles from weedy radish enhance the florigenic potential of rootstocks for graft-mediated floral induction in Brassicaceae.
    Article Snippet: A total of 15 μ g of extracted protein was separated on a 15% (w/v) polyacrylamide gel (e-PAGEL E-R15L; ATTO Corp., Japan) and transferred to PVDF membranes using the Trans-Blot Turbo system (Trans-Blot Turbo RTA Transfer Kit, Bio-Rad Laboratories, Inc., USA). .. After transfer, membranes were stained with No-Stain TM Protein Labeling Reagent (Thermo Fisher Scientific Inc., USA), blocked with PBST (phosphate-buffered saline containing 0.1% Tween 20) containing 1% skim milk, and incubated with an anti-FT antibody ( Motoki et al. 2022) diluted to 300 ng mL − 1 in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd). .. Membranes were then incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:20 000 dilution; Cell Signaling Technology, Inc., USA) in Can Get Signal Solution 2 (Toyobo Co., Ltd).

    Article Title: KRAS (G12D)‑selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal® (Toyobo Co., Ltd.), washed thrice using Tris‐buffered saline‐Tween‐20 (TBS‐T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal® Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti‐phospho‐ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti‐ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti‐α‐actin antibody (Sigma‐Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS‐T and incubated with HRP‐labeled anti‐rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal® Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS‐T, incu‐ bated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge‐coupled device system (ImageQuantTM; LAS‐4000mini; Cytiva).

    Article Title: Apoptosis- and ferroptosis-inducing activity of triptolide in SBC-3 human small-cell lung cancer cells.
    Article Snippet: 1 Laboratory of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan Abstract Small-cell lung cancer (SCLC) is an aggressive malignancy characterized by rapid progression, poor prognosis, and a high recurrence rate following chemotherapy.. Despite recent advances in immunotherapy, treatment options remain limited, underscoring the urgent need for novel anticancer agents.. Triptolide, a natural diterpenoid, has been reported to exhibit cytotoxic activity against various cancer cell lines.

    Article Title: Plantago lanceolata extract inhibits porcine epidemic diarrhea virus and emerging enveloped viruses by interfering with virus internalization in vitro.
    Article Snippet: Ethnopharmacological relevance: Plantago lanceolata is a widely distributed perennial herb that has traditionally been used for the treatment of gastrointestinal disorders and upper respiratory tract diseases, as well as for wound and skin care.. However, its antiviral activity against porcine epidemic diarrhea virus (PEDV) has not yet

    Article Title: Bioactive potential of 70% ethanol extracts from different cheese products: LPS-stimulated macrophage cytokine response, radical-scavenging activity, and peptide characterization
    Article Snippet: .. After three washes in PBST, the membranes were incubated with primary antibodies suspended in Can Get Signal Immunoreaction Enhancer Solution 1 (TOYOBO Co. Ltd., Osaka, Japan). .. The primary antibodies used in this study were anti-NF-κB p65 (# 8242, Cell Signaling Technology, Inc., Beverly, MA, USA), anti-phospho-IκB-α (# 2859, Cell Signaling Technology), anti-IκB-α (# 4812, Cell Signaling Technology), anti-phosphop38 MAPK (# 4511, Cell Signaling Technology), anti-p38 MAPK (# 8690, Cell Signaling Technology), anti-phospho-SAPK/JNK (# 4668, Cell Signaling Technology), and anti-SAPK/JNK (# 9252, Cell Signaling Technology).

    Blocking Assay:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal (Toyobo Co., Ltd.), washed thrice using Tris-buffered saline-Tween-20 (TBS-T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti-phospho-ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti-ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti-α-actin antibody (Sigma-Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS-T and incubated with HRP-labeled anti-rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS-T, incubated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge-coupled device system (ImageQuant; LAS-4000mini; Cytiva).

    Article Title: KRAS (G12D)‑selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal® (Toyobo Co., Ltd.), washed thrice using Tris‐buffered saline‐Tween‐20 (TBS‐T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal® Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti‐phospho‐ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti‐ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti‐α‐actin antibody (Sigma‐Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS‐T and incubated with HRP‐labeled anti‐rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal® Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS‐T, incu‐ bated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge‐coupled device system (ImageQuantTM; LAS‐4000mini; Cytiva).

    Article Title: Apoptosis- and ferroptosis-inducing activity of triptolide in SBC-3 human small-cell lung cancer cells.
    Article Snippet: 1 Laboratory of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1, Horinouchi, Hachioji, Tokyo 192-0392, Japan Abstract Small-cell lung cancer (SCLC) is an aggressive malignancy characterized by rapid progression, poor prognosis, and a high recurrence rate following chemotherapy.. Despite recent advances in immunotherapy, treatment options remain limited, underscoring the urgent need for novel anticancer agents.. Triptolide, a natural diterpenoid, has been reported to exhibit cytotoxic activity against various cancer cell lines.

    Saline:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal (Toyobo Co., Ltd.), washed thrice using Tris-buffered saline-Tween-20 (TBS-T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti-phospho-ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti-ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti-α-actin antibody (Sigma-Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS-T and incubated with HRP-labeled anti-rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS-T, incubated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge-coupled device system (ImageQuant; LAS-4000mini; Cytiva).

    Article Title: Far-red-enriched ultra-long-day conditions induce constitutive FT expression and rapid flowering in radish rootstocks, promoting graft-mediated floral induction in Brassicaceae crops
    Article Snippet: After transfer, membranes were stained with Ponceau S solution (Beacle, Inc., Japan) and scanned to quantify total protein loading for normalization. .. Membranes were blocked with PBST (phosphate-buffered saline containing 0.1% Tween 20) containing 1% skim milk for 1 h at room temperature (20–25◦C), then incubated with an anti-FT antibody (Motoki et al., 2022) diluted to 300 ng mL−1 in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.). .. Subsequently, membranes were incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:20,000 dilution; Cell Signaling Technology, Inc., USA) in Can Get Signal Solution 2 (Toyobo Co., Ltd.) at room temperature.

    Article Title: Disrupted FLOWERING LOCUS C alleles from weedy radish enhance the florigenic potential of rootstocks for graft-mediated floral induction in Brassicaceae.
    Article Snippet: A total of 15 μ g of extracted protein was separated on a 15% (w/v) polyacrylamide gel (e-PAGEL E-R15L; ATTO Corp., Japan) and transferred to PVDF membranes using the Trans-Blot Turbo system (Trans-Blot Turbo RTA Transfer Kit, Bio-Rad Laboratories, Inc., USA). .. After transfer, membranes were stained with No-Stain TM Protein Labeling Reagent (Thermo Fisher Scientific Inc., USA), blocked with PBST (phosphate-buffered saline containing 0.1% Tween 20) containing 1% skim milk, and incubated with an anti-FT antibody ( Motoki et al. 2022) diluted to 300 ng mL − 1 in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd). .. Membranes were then incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:20 000 dilution; Cell Signaling Technology, Inc., USA) in Can Get Signal Solution 2 (Toyobo Co., Ltd).

    Article Title: KRAS (G12D)‑selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal® (Toyobo Co., Ltd.), washed thrice using Tris‐buffered saline‐Tween‐20 (TBS‐T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal® Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti‐phospho‐ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti‐ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti‐α‐actin antibody (Sigma‐Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS‐T and incubated with HRP‐labeled anti‐rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal® Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS‐T, incu‐ bated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge‐coupled device system (ImageQuantTM; LAS‐4000mini; Cytiva).

    Article Title: Plantago lanceolata extract inhibits porcine epidemic diarrhea virus and emerging enveloped viruses by interfering with virus internalization in vitro.
    Article Snippet: Ethnopharmacological relevance: Plantago lanceolata is a widely distributed perennial herb that has traditionally been used for the treatment of gastrointestinal disorders and upper respiratory tract diseases, as well as for wound and skin care.. However, its antiviral activity against porcine epidemic diarrhea virus (PEDV) has not yet

    Transcranial Magnetic Stimulation:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal (Toyobo Co., Ltd.), washed thrice using Tris-buffered saline-Tween-20 (TBS-T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti-phospho-ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti-ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti-α-actin antibody (Sigma-Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS-T and incubated with HRP-labeled anti-rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS-T, incubated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge-coupled device system (ImageQuant; LAS-4000mini; Cytiva).

    Cell-Signaling:

    Article Title: KRAS (G12D)-selective inhibitor MRTX1133 suppresses proliferation and differentially modulates chemosensitivity in ovarian mucinous carcinoma
    Article Snippet: .. Membranes were incubated at room temperature for 1 h in PVDF Blocking Reagent for Can Get Signal (Toyobo Co., Ltd.), washed thrice using Tris-buffered saline-Tween-20 (TBS-T) and incubated overnight with the following antibodies diluted 1:1,000 at room temperature in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd.): Anti-phospho-ERK (D13.14.4E; Cell Signaling Technology, Inc., cat. no. 4370), anti-ERK (137F5; Cell Signaling Technology, Inc., cat. no. 4695) and anti-α-actin antibody (Sigma-Aldrich; Merck KgaA, cat. no. A2066). .. After the reaction, membranes were washed three times with TBS-T and incubated with HRP-labeled anti-rabbit antibodies (Rabbit IgG HRP Linked F(ab')2; cat. no. NA9340V; Cytiva) diluted 1:1,000 in Can Get Signal Immunoreaction Enhancer Solution 2 (Toyobo Co., Ltd.) at room temperature for 1 h. Membranes were then washed three times with TBS-T, incubated with ECL Prime Western Blotting Detection reagent (Cytiva) and imaged using a cooled charge-coupled device system (ImageQuant; LAS-4000mini; Cytiva).

    Staining:

    Article Title: Disrupted FLOWERING LOCUS C alleles from weedy radish enhance the florigenic potential of rootstocks for graft-mediated floral induction in Brassicaceae.
    Article Snippet: A total of 15 μ g of extracted protein was separated on a 15% (w/v) polyacrylamide gel (e-PAGEL E-R15L; ATTO Corp., Japan) and transferred to PVDF membranes using the Trans-Blot Turbo system (Trans-Blot Turbo RTA Transfer Kit, Bio-Rad Laboratories, Inc., USA). .. After transfer, membranes were stained with No-Stain TM Protein Labeling Reagent (Thermo Fisher Scientific Inc., USA), blocked with PBST (phosphate-buffered saline containing 0.1% Tween 20) containing 1% skim milk, and incubated with an anti-FT antibody ( Motoki et al. 2022) diluted to 300 ng mL − 1 in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd). .. Membranes were then incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:20 000 dilution; Cell Signaling Technology, Inc., USA) in Can Get Signal Solution 2 (Toyobo Co., Ltd).

    Labeling:

    Article Title: Disrupted FLOWERING LOCUS C alleles from weedy radish enhance the florigenic potential of rootstocks for graft-mediated floral induction in Brassicaceae.
    Article Snippet: A total of 15 μ g of extracted protein was separated on a 15% (w/v) polyacrylamide gel (e-PAGEL E-R15L; ATTO Corp., Japan) and transferred to PVDF membranes using the Trans-Blot Turbo system (Trans-Blot Turbo RTA Transfer Kit, Bio-Rad Laboratories, Inc., USA). .. After transfer, membranes were stained with No-Stain TM Protein Labeling Reagent (Thermo Fisher Scientific Inc., USA), blocked with PBST (phosphate-buffered saline containing 0.1% Tween 20) containing 1% skim milk, and incubated with an anti-FT antibody ( Motoki et al. 2022) diluted to 300 ng mL − 1 in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Co., Ltd). .. Membranes were then incubated with horseradish peroxidase-conjugated anti-rabbit IgG (1:20 000 dilution; Cell Signaling Technology, Inc., USA) in Can Get Signal Solution 2 (Toyobo Co., Ltd).



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